Vol 7: Production of marker-free transgenic Jatropha curcas expressing hybrid Bacillus thuringiensis δ-endotoxin Cry1Ab-1Ac for resistance to larvae of tortrix moth Archips micaceanus.Report as inadecuate



 Vol 7: Production of marker-free transgenic Jatropha curcas expressing hybrid Bacillus thuringiensis δ-endotoxin Cry1Ab-1Ac for resistance to larvae of tortrix moth Archips micaceanus.


Vol 7: Production of marker-free transgenic Jatropha curcas expressing hybrid Bacillus thuringiensis δ-endotoxin Cry1Ab-1Ac for resistance to larvae of tortrix moth Archips micaceanus. - Download this document for free, or read online. Document in PDF available to download.

Download or read this book online for free in PDF: Vol 7: Production of marker-free transgenic Jatropha curcas expressing hybrid Bacillus thuringiensis δ-endotoxin Cry1Ab-1Ac for resistance to larvae of tortrix moth Archips micaceanus.
This article is from Biotechnology for Biofuels, volume 7.AbstractBackground: The potential biofuel plant Jatropha curcas L. is affected by larvae of Archips micaceanus Walker, a moth of the family Tortricidae. The hybrid Bacillus thuringiensis Bt δ-endotoxin protein Cry1Ab-1Ac confers resistance to lepidopteran insects in transgenic rice. Results: Here, we report the production of a marker-free transgenic line of J. curcas L10 expressing Cry1Ab-1Ac using Agrobacterium-mediated transformation and a chemically regulated, Cre-loxP-mediated DNA recombination system. L10 carries a single copy of marker-free T-DNA that contains the Cry1Ab-1Ac gene under the control of a maize phosphoenolpyruvate carboxylase gene promoter PPepc:Cry1Ab-1Ac:TNos. The PPepc:Cry1Ab-1Ac:TNos gene was highly expressed in leaves of L10 plants. Insecticidal bioassays using leaf explants of L10 resulted in 80-100% mortality of larvae of A. micaceanus at 4 days after infestation. Conclusion: The results demonstrate that the hybrid Bt δ-endotoxin protein Cry1Ab-1Ac expressed in Jatropha curcas displays strong insecticidal activity to A. micaceanus. The marker-free transgenic J. curcas line L10 can be used for breeding of insect resistance to A. micaceanus.



Author: Gu, Keyu; Mao, Huizhu; Yin, Zhongchao

Source: https://archive.org/







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