Vol 9: Porous Silk Scaffolds for Delivery of Growth Factors and Stem Cells to Enhance Bone Regeneration.Report as inadecuate



 Vol 9: Porous Silk Scaffolds for Delivery of Growth Factors and Stem Cells to Enhance Bone Regeneration.


Vol 9: Porous Silk Scaffolds for Delivery of Growth Factors and Stem Cells to Enhance Bone Regeneration. - Download this document for free, or read online. Document in PDF available to download.

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This article is from PLoS ONE, volume 9.AbstractStem cell-based tissue engineering shows promise for bone regeneration and requires artificial microenvironments to enhance the survival, proliferation and differentiation of the seeded cells. Silk fibroin, as a natural protein polymer, has unique properties for tissue regeneration. The present study aimed to evaluate the influence of porous silk scaffolds on rat bone marrow stem cells BMSCs by lenti-GFP tracking both in vitro and in vivo in cranial bone defects. The number of cells seeded within silk scaffolds in rat cranial bone defects increased from 2 days to 2 weeks after implantation, followed by a decrease at eight weeks. Importantly, the implanted cells survived for 8 weeks in vivo and some of the cells might differentiate into endothelial cells and osteoblasts induced by the presence of VEGF and BMP-2 in the scaffolds to promote angiogenesis and osteogenesis. The results demonstrate that porous silk scaffolds provide a suitable niche to maintain long survival and function of the implanted cells for bone regeneration.



Author: Zhang, Wenjie; Zhu, Chao; Ye, Dongxia; Xu, Ling; Zhang, Xiaochen; Wu, Qianju; Zhang, Xiuli; Kaplan, David L.; Jiang, Xinquan

Source: https://archive.org/







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