Vol 9: Plasma Autoantibodies against Heat Shock Protein 70, Enolase 1 and Ribonuclease-Angiogenin Inhibitor 1 as Potential Biomarkers for Cholangiocarcinoma.Report as inadecuate



 Vol 9: Plasma Autoantibodies against Heat Shock Protein 70, Enolase 1 and Ribonuclease-Angiogenin Inhibitor 1 as Potential Biomarkers for Cholangiocarcinoma.


Vol 9: Plasma Autoantibodies against Heat Shock Protein 70, Enolase 1 and Ribonuclease-Angiogenin Inhibitor 1 as Potential Biomarkers for Cholangiocarcinoma. - Download this document for free, or read online. Document in PDF available to download.

Download or read this book online for free in PDF: Vol 9: Plasma Autoantibodies against Heat Shock Protein 70, Enolase 1 and Ribonuclease-Angiogenin Inhibitor 1 as Potential Biomarkers for Cholangiocarcinoma.
This article is from PLoS ONE, volume 9.AbstractThe diagnosis of cholangiocarcinoma CCA is often challenging, leading to poor prognosis. CCA arises via chronic inflammation which may be associated with autoantibodies production. This study aims to identify IgG antibodies directed at self-proteins and tumor-associated antigens. Proteins derived from immortalized cholangiocyte cell line MMNK1 and CCA cell lines M055, M214 and M139 were separated using 2-dimensional electrophoresis and incubated with pooled plasma of patients with CCA and non-neoplastic controls by immunoblotting. Twenty five immunoreactive spots against all cell lines-derived proteins were observed on stained gels and studied by LC-MS-MS. Among these, heat shock protein 70 HSP70, enolase 1 ENO1 and ribonuclease-angiogenin inhibitor 1 RNH1 obtained the highest matching scores and were thus selected for further validation. Western blot revealed immunoreactivity against HSP70 and RNH1 in the majority of CCA cases and weakly in healthy individuals. Further, ELISA showed that plasma HSP70 autoantibody level in CCA was significantly capable to discriminate CCA from healthy individuals with an area under the receiver operating characteristic curve of 0.9158 cut-off 0.2630, 93.55% sensitivity and 73.91% specificity. Plasma levels of IgG autoantibodies against HSP70 were correlated with progression from healthy individuals to cholangitis to CCA r = 0.679, P



Author: Rucksaken, Rucksak; Pairojkul, Chawalit; Pinlaor, Porntip; Khuntikeo, Narong; Roytrakul, Sittiruk; Selmi, Carlo; Pinlaor, Somchai

Source: https://archive.org/







Related documents