Vol 15: Matrilin-3 Chondrodysplasia Mutations Cause Attenuated Chondrogenesis, Premature Hypertrophy and Aberrant Response to TGF- in Chondroprogenitor Cells.Report as inadecuate



 Vol 15: Matrilin-3 Chondrodysplasia Mutations Cause Attenuated Chondrogenesis, Premature Hypertrophy and Aberrant Response to TGF- in Chondroprogenitor Cells.


Vol 15: Matrilin-3 Chondrodysplasia Mutations Cause Attenuated Chondrogenesis, Premature Hypertrophy and Aberrant Response to TGF- in Chondroprogenitor Cells. - Download this document for free, or read online. Document in PDF available to download.

Download or read this book online for free in PDF: Vol 15: Matrilin-3 Chondrodysplasia Mutations Cause Attenuated Chondrogenesis, Premature Hypertrophy and Aberrant Response to TGF- in Chondroprogenitor Cells.
This article is from International Journal of Molecular Sciences, volume 15.AbstractStudies have shown that mutations in the matrilin-3 gene MATN3 are associated with multiple epiphyseal dysplasia MED and spondyloepimetaphyseal dysplasia SEMD. We tested whether MATN3 mutations affect the differentiation of chondroprogenitor and-or mesenchymal stem cells, which are precursors to chondrocytes. ATDC5 chondroprogenitors stably expressing wild-type WT MATN3 underwent spontaneous chondrogenesis. Expression of chondrogenic markers collagen II and aggrecan was inhibited in chondroprogenitors carrying the MED or SEMD MATN3 mutations. Hypertrophic marker collagen X remained attenuated in WT MATN3 chondroprogenitors, whereas its expression was elevated in chondroprogenitors expressing the MED or SEMD mutant MATN3 gene suggesting that these mutations inhibit chondrogenesis but promote hypertrophy. TGF-β treatment failed to rescue chondrogenesis markers but dramatically increased collagen X mRNA expression in mutant MATN3 expressing chondroprogenitors. Synovium derived mesenchymal stem cells harboring the SEMD mutation exhibited lower glycosaminoglycan content than those of WT MATN3 in response to TGF-β. Our results suggest that the properties of progenitor cells harboring MATN3 chondrodysplasia mutations were altered, as evidenced by attenuated chondrogenesis and premature hypertrophy. TGF-β treatment failed to completely rescue chondrogenesis but instead induced hypertrophy in mutant MATN3 chondroprogenitors. Our data suggest that chondroprogenitor cells should be considered as a potential target of chondrodysplasia therapy.



Author: Jayasuriya, Chathuraka T.; Zhou, Fiona H.; Pei, Ming; Wang, Zhengke; Lemme, Nicholas J.; Haines, Paul; Chen, Qian

Source: https://archive.org/



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