Vol 26: Kisspeptin-10 Enhanced Egg Production in Quails Associated with the Increase of Triglyceride Synthesis in Liver.Report as inadecuate



 Vol 26: Kisspeptin-10 Enhanced Egg Production in Quails Associated with the Increase of Triglyceride Synthesis in Liver.


Vol 26: Kisspeptin-10 Enhanced Egg Production in Quails Associated with the Increase of Triglyceride Synthesis in Liver. - Download this document for free, or read online. Document in PDF available to download.

Download or read this book online for free in PDF: Vol 26: Kisspeptin-10 Enhanced Egg Production in Quails Associated with the Increase of Triglyceride Synthesis in Liver.
This article is from Asian-Australasian Journal of Animal Sciences, volume 26.AbstractOur previous results showed that kisspeptin-10 Kp-10 injections via intraperitoneal i.p. once daily for three weeks notably promoted the egg laying rate in quails. In order to investigate the mechanism behind the effects of Kp-10 on enhancing the egg laying rate in birds, this study focused on the alternations of lipids synthesis in liver after Kp-10 injections. 75 female quails 22 d of age were allocated to three groups randomly, and subjected to 0 control, Con, 10 nmol low dosage, L and 100 nmol high dosage, H Kp-10 injections via i.p. once daily for three weeks, respectively. At d 52, quails were sacrificed and sampled for further analyses. Serum E2 concentration was increased by Kp-10 injections, and reached statistical significance in H group. Serum triglyceride TG concentrations were increased by 46.7% in L group and 36.8% in H group, respectively, but did not reach statistical significance, and TG contents in liver were significantly elevated by Kp-10 injections in a dose-dependent manner. Serum total cholesterol Tch concentrations significantly decreased in H group, while in H group the hepatic Tch content was markedly increased. The level of non-esterified fatty acid NEFA, apolipoprotein A1 and B apoA1 and apoB were not altered by Kp-10 injections. The genes expression of sterol regulatory element binding protein-1 SREBP-1, fatty acid synthetase FAS, apolipoprotein VLDL-II apoVLDL-II, cholesterol 7α-hydroxylase CYP7A1 and vitellogenin II VTG-II were significantly up-regulated by high but not low dosage of Kp-10 injection compared to the control group. However, the expression of SREBP-2, acetyl-CoA carboxylase ACCα, malic enzyme ME, stearoyl-CoA Δ9 desaturase 1 SCD1, apolipoprotein A1 apoA1, fatty acid binding protein 2 FABP2, 3-hydroxyl-3-methyl glutaryl-coenzyme A reductases HMGCR, estrogen receptor α, β ERα and β mRNA were not affected by Kp-10 treatment. In line with hepatic mRNA abundance, hepatic SREBP1 protein content was significantly higher in H group. Although the mRNA expression was not altered, the content of ERα protein in liver was also significantly increased in H group. However, SREBP-2 protein content in liver was not changed by Kp-10 treatment. In conclusion, exogenous Kp-10 consecutive injections during juvenile stage significantly advanced the tempo of egg laying in quails, which was associated with the significant elevation in hepatic lipids synthesis and transport.



Author: Wu, J.; Fu, W.; Huang, Y.; Ni, Y.; Zhao, R.

Source: https://archive.org/







Related documents