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Julio Enrique Duarte ; Joseph Samitier Martí ;Ingeniería e Investigación 2008, 28 3

Author: Flavio Humberto Fernández Morales

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Ingeniería e Investigación ISSN: 0120-5609 revii_bog@unal.edu.co Universidad Nacional de Colombia Colombia Fernández Morales, Flavio Humberto; Duarte, Julio Enrique; Samitier Martí, Joseph Non-uniform electric field-induced yeast cell electrokinetic behavior Ingeniería e Investigación, vol.
28, núm.
3, diciembre, 2008, pp.
116-121 Universidad Nacional de Colombia Bogotá, Colombia Available in: http:--www.redalyc.org-articulo.oa?id=64328317 How to cite Complete issue More information about this article Journals homepage in redalyc.org Scientific Information System Network of Scientific Journals from Latin America, the Caribbean, Spain and Portugal Non-profit academic project, developed under the open access initiative REVISTA INGENIERÍA E INVESTIGACIÓN VOL.
28 No.
3, DICIEMBRE DE 2008 (116-121) Non-uniform electric field-induced yeast cell electrokinetic behavior Comportamiento electrocinético de levaduras inducido por campos eléctricos no uniformes Flavio Humberto Fernández Morales1, Julio Enrique Duarte2 y Joseph Samitier Martí3 ABSTRACT Common dielectrophoresis (c-DEP, i.e.
neutral matter motion induced by non-uniform electric fields) has become a basic phenomenon of biochips intended for medical, biological and chemical assays, especially when they imply bioparticle handling.
This paper deals with modelling and experimental verification of a castellated, c-DEP-based, microelectrode array intended to handle biological objects.
The proposed microsystem was developed employing platinum electrodes patterned by lift-off, silicon micromachining and photoresin patterning techniques.
Saccharomyces cerevisiae were used as test bioparticles for experimental verification.
Yeast cells were repelled toward electrode bays and toward interelectrodic gaps tor frequencies around 20 MHz where there is minimum electric field strength, corresponding to a negative dielectrophoretic phenomenon.
Yeast cell agglomerations were observed around electrode edges for f...





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