Vol 7: Inhibition of Aurora-B suppresses osteosarcoma cell migration and invasion.Report as inadecuate



 Vol 7: Inhibition of Aurora-B suppresses osteosarcoma cell migration and invasion.


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This article is from Experimental and Therapeutic Medicine, volume 7.AbstractPrevious studies have suggested that Aurora-B may be involved in cancer metastasis. However, its role has been poorly evaluated in osteosarcoma OS. The aim of this study was to investigate the correlation between Aurora-B expression and metastasis in human OS. The human OS cell line, U2-OS, and OS biopsy specimens were used in the study. The expression of Aurora-B protein was examined using immunohistochemistry and western blotting in OS tissues and U2-OS cells, respectively. AZD1152-hydroxyquinazoline-pyrazol-anilide, an inhibitor of Aurora-B, was used to inhibit Aurora-B expression in U2-OS cells. The effect of Aurora-B inhibition on U2-OS cell proliferation, invasion and migration was assessed using MTT, colony formation, wound healing and Transwell assays. The results showed that positive expression of the Aurora-B protein was observed in the nucleus, and that Aurora-B expression levels in the cases with pulmonary metastases were significantly higher than in those without metastasis. In vitro, the proliferation, invasion and migration of U2-OS cells were suppressed by the inhibition of Aurora-B. These results suggest that Aurora-B may be involved in OS metastasis, and may be a promising target in the treatment of OS metastasis.



Author: ZHU, XIAO PING; LIU, ZHI LI; PENG, AI FEN; ZHOU, YUN FEI; LONG, XIN HUA; LUO, QING FENG; HUANG, SHAN HU; SHU, YONG

Source: https://archive.org/







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