Vol 9: Impact of Age on the Cerebrovascular Proteomes of Wild-Type and Tg-SwDI Mice.Report as inadecuate



 Vol 9: Impact of Age on the Cerebrovascular Proteomes of Wild-Type and Tg-SwDI Mice.


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This article is from PLoS ONE, volume 9.AbstractThe structural integrity of cerebral vessels is compromised during ageing. Abnormal amyloid Aβ deposition in the vasculature can accelerate age-related pathologies. The cerebrovascular response associated with ageing and microvascular Aβ deposition was defined using quantitative label-free shotgun proteomic analysis. Over 650 proteins were quantified in vessel-enriched fractions from the brains of 3 and 9 month-old wild-type WT and Tg-SwDI mice. Sixty-five proteins were significantly increased in older WT animals and included several basement membrane proteins nidogen-1, basement membrane-specific heparan sulfate proteoglycan core protein, laminin subunit gamma-1 precursor and collagen alpha-2IV chain preproprotein. Twenty-four proteins were increased and twenty-one decreased in older Tg-SwDI mice. Of these, increases in Apolipoprotein E APOE and high temperature requirement serine protease-1 HTRA1 and decreases in spliceosome and RNA-binding proteins were the most prominent. Only six shared proteins were altered in both 9-month old WT and Tg-SwDI animals. The age-related proteomic response in the cerebrovasculature was distinctly different in the presence of microvascular Aβ deposition. Proteins found differentially expressed within the WT and Tg-SwDI animals give greater insight to the mechanisms behind age-related cerebrovascular dysfunction and pathologies and may provide novel therapeutic targets.



Author: Searcy, James L.; Le Bihan, Thierry; Salvadores, Natalia; McCulloch, James; Horsburgh, Karen

Source: https://archive.org/



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