Functional cooperation of of IL-1β and RGS4 in the brachial plexus avulsion mediated brain reorganizationReport as inadecuate




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Journal of Brachial Plexus and Peripheral Nerve Injury

, 5:18

First Online: 07 December 2010Received: 04 June 2010Accepted: 07 December 2010

Abstract

BackgroundsThere is considerable evidence that central nervous system is continuously modulated by activity, behavior and skill acquisition. This study is to examine the reorganization in cortical and subcortical regions in response to brachial plexus avulsion.

MethodsAdult C57BL-6 mice were divided into four groups: control, 1, 3 and 6 month of brachial plexus avulsion. IL-1β, IL-6 and RGS4 expression in cortex, brainstem and spinal cord were detected by BiostarM-140 s microarray and real-time PCR. RGS4 subcellular distribution and modulation were further analyzed by primary neuron culture and Western Blot.

ResultsAfter 1, 3 and 6 months of brachial plexus avulsion, 49 0 up, 49 down, 29 17 up, 12 down, 13 9 up, 4 down genes in cerebral cortex, 40 8 up, 32 down, 11 7 up, 4 down, 137 63 up, 74 down in brainstem, 27 14 up, 13 down, 33 18 up, 15 down, 60 29 up, 31 down in spinal cord were identified. Among the regulated gene, IL-1β and IL-6 were sustainable enhanced in brain stem, while PKACβ and RGS4 were up-regulated throughout cerebral cortex, brainstem and spinal cord in 3 and 6 month of nerve injury. Intriguingly, subcellular distribution of RGS4 in above three regions was dependent on the functional correlation of PKA and IL-1β.

ConclusionData herein indicated that brachial plexus avulsion could efficiently initiate and perpetuate the brain reorganization. Network involved IL-1β and RGS4 signaling might implicate in the re-establish and strengthening of the local circuits at the cortical and subcortical levels.

AbbreviationscAMPcyclic AMP

CNScentral nervous system

CREBcAMP response element-binding protein

GAPGTPase activating protein

IL-1βinterleukin 1-β

IL-6interleukin-6

PKACβprotein kinase A catalytic subunit β

RGS4regulator of G protein signaling 4

Electronic supplementary materialThe online version of this article doi:10.1186-1749-7221-5-18 contains supplementary material, which is available to authorized users.

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Author: Jifeng Li - Hui Zhao - Pengbo Luo - Yudong Gu

Source: https://link.springer.com/







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