A DNA method for qualitative identification of plant raw materials in feedstuffReport as inadecuate




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European Food Research and Technology

, Volume 229, Issue 3, pp 475–484

First Online: 13 May 2009Received: 12 January 2009Revised: 22 April 2009Accepted: 27 April 2009

Abstract

This research developed a simple and not expensive DNA method for the qualitative identification of plant raw materials used as feed mixtures. Specific simple sequence tagged STS markers were developed to detect faba bean Lectin A gene, field pea Convicilin A gene, grain sorghum UDP-glucosyltransferase gene and barley Hordoindoline-a gene, whereas identification of durum and common wheat lipid transfer protein gene, soybean Gly m Bd 30K allergen gene and maize invertase gene was carried out using markers available from the literature. Cross-reactivity of the primer pairs was also checked against oat, rye, kidney bean and lentil. The method was effectively applied to the analysis of flour mixtures and extruded feedstuff. It could be included in traceability and certification of animal feeding systems within high quality animal production chains which are strictly related to the production area by the valorisation of locally grown raw materials.

KeywordsTraceability Feed Molecular markers STS PCR  Download fulltext PDF



Author: Stefano Tavoletti - Linda Iommarini - Marina Pasquini

Source: https://link.springer.com/







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