An ultrasoft X-ray multi-microbeam irradiation system for studies of DNA damage responses by fixed- and live-cell fluorescence microscopyReport as inadecuate




An ultrasoft X-ray multi-microbeam irradiation system for studies of DNA damage responses by fixed- and live-cell fluorescence microscopy - Download this document for free, or read online. Document in PDF available to download.

European Biophysics Journal

, Volume 38, Issue 6, pp 721–728

First Online: 03 June 2009Received: 27 February 2009Revised: 20 April 2009Accepted: 29 April 2009

Abstract

Localized induction of DNA damage is a valuable tool for studying cellular DNA damage responses. In recent decades, methods have been developed to generate DNA damage using radiation of various types, including photons and charged particles. Here we describe a simple ultrasoft X-ray multi-microbeam system for high dose-rate, localized induction of DNA strand breaks in cells at spatially and geometrically adjustable sites. Our system can be combined with fixed- and live-cell microscopy to study responses of cells to DNA damage.

KeywordsSoft X-rays Microbeam irradiation DNA damage DNA double-strand breaks Live-cell microscopy This article has been submitted as a contribution to the Festschrift entitled -Uncovering cellular sub-structures by light microscopy- in honour of Professor Cremer’s 65th birthday.

C. van Oven and P. M. Krawczyk contributed equally to this work.

Download fulltext PDF



Author:

Source: https://link.springer.com/



DOWNLOAD PDF




Related documents