Transcriptome analysis of Taenia solium cysticerci using Open Reading Frame ESTs ORESTESReport as inadecuate




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Parasites and Vectors

, 2:35

First Online: 31 July 2009Received: 05 May 2009Accepted: 31 July 2009

Abstract

BackgroundHuman infection by the pork tapeworm Taenia solium affects more than 50 million people worldwide, particularly in underdeveloped and developing countries. Cysticercosis which arises from larval encystation can be life threatening and difficult to treat. Here, we investigate for the first time the transcriptome of the clinically relevant cysticerci larval form.

ResultsUsing Expressed Sequence Tags ESTs produced by the ORESTES method, a total of 1,520 high quality ESTs were generated from 20 ORESTES cDNA mini-libraries and its analysis revealed fragments of genes with promising applications including 51 ESTs matching antigens previously described in other species, as well as 113 sequences representing proteins with potential extracellular localization, with obvious applications for immune-diagnosis or vaccine development.

ConclusionThe set of sequences described here will contribute to deciphering the expression profile of this important parasite and will be informative for the genome assembly and annotation, as well as for studies of intra- and inter-specific sequence variability. Genes of interest for developing new diagnostic and therapeutic tools are described and discussed.

AbbreviationsESTsExpressed Sequence Tags

ORESTESOpen Reading frame Expressed Sequence Tags

PCRPolymerase Chain Reaction.

Electronic supplementary materialThe online version of this article doi:10.1186-1756-3305-2-35 contains supplementary material, which is available to authorized users.

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Author: Carolina R Almeida - Patricia H Stoco - Glauber Wagner - Thaís CM Sincero - Gianinna Rotava - Ethel Bayer-Santos - Julia

Source: https://link.springer.com/







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