2-DE analysis indicates that Acinetobacter baumannii displays a robust and versatile metabolismReport as inadecuate




2-DE analysis indicates that Acinetobacter baumannii displays a robust and versatile metabolism - Download this document for free, or read online. Document in PDF available to download.

Proteome Science

, 7:37

First Online: 28 September 2009Received: 29 June 2009Accepted: 28 September 2009

Abstract

BackgroundAcinetobacter baumannii is a nosocomial pathogen that has been associated with outbreak infections in hospitals. Despite increasing awareness about this bacterium, its proteome remains poorly characterised, however recently the complete genome of A. baumannii reference strain ATCC 17978 has been sequenced. Here, we have used 2-DE and MALDI-TOF-TOF approach to characterise the proteome of this strain.

ResultsThe membrane and cytoplasmatic protein extracts were analysed separately, these analyses revealed the reproducible presence of 239 and 511 membrane and cytoplamatic protein spots, respectively. MALDI-TOF-TOF characterisation identified a total of 192 protein spots 37 membrane and 155 cytoplasmatic and revealed that the identified membrane proteins were mainly transport-related proteins, whereas the cytoplasmatic proteins were of diverse nature, although mainly related to metabolic processes.

ConclusionThis work indicates that A. baumannii has a versatile and robust metabolism and also reveal a number of proteins that may play a key role in the mechanism of drug resistance and virulence. The data obtained complements earlier reports of A. baumannii proteome and provides new tools to increase our knowledge on the protein expression profile of this pathogen.

Abbreviations2-DEtwo-dimensional gel electrophoresis

Hspheat-shock protein

MALDImatrix-assisted laser desorption ionisation

Ompouter membrane protein

pAsputative alien islands

TOFtime of flight.

Electronic supplementary materialThe online version of this article doi:10.1186-1477-5956-7-37 contains supplementary material, which is available to authorized users.

Download fulltext PDF



Author: Nelson C Soares - Maria P Cabral - José R Parreira - Carmen Gayoso - Maria J Barba - Germán Bou

Source: https://link.springer.com/



DOWNLOAD PDF




Related documents