Proteomic analysis of regenerating mouse liver following 50% partial hepatectomyReport as inadecuate




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Proteome Science

, 7:48

First Online: 29 December 2009Received: 03 August 2009Accepted: 29 December 2009

Abstract

BackgroundAlthough 70% or 2-3 partial hepatectomy PH is the most studied model for liver regeneration, the hepatic protein expression profile associated with lower volume liver resection such as 50% PH has not yet been reported. Therefore, the aim of this study was to determine the global protein expression profile of the regenerating mouse liver following 50% PH by differential proteomics, and thereby gaining some insights into the hepatic regeneration mechanisms under this milder but clinically more relevant condition.

ResultsProteins from sham-operated mouse livers and livers regenerating for 24 h after 50% PH were separated by SDS-PAGE and analyzed by nanoUPLC-Q-Tof mass spectrometry. Compared to sham-operated group, there were totally 87 differentially expressed proteins with 50 up-regulated and 37 down-regulated ones identified in the regenerating mouse livers, most of which have not been previously related to liver regeneration. Remarkably, over 25 differentially expressed proteins were located at mitochondria. Several of the mitochondria-resident proteins which play important roles in citric acid cycle, oxidative phosphorylation and ATP production were found to be down-regulated, consistent with the recently-proposed model in which the reduction of ATP content in the remnant liver gives rise to early stress signals that contribute to the onset of liver regeneration. Pathway analysis revealed a central role of c-Myc in the regulation of liver regeneration.

ConclusionsOur study provides novel evidence for mitochondria as a pivotal organelle that is connected to liver regeneration, and lays the foundation for further studies on key factors and pathways involved in liver regeneration following 50% PH, a condition frequently used for partial liver transplantation and conservative liver resection.

AbbreviationsPHpartial hepatectomy

SLTsplit-liver transplantation

PMSFpheylmethylsulfonyl fluoride

EDTAethylene diamine tetraacetic acid

PBSphosphate buffered saline

SDS-PAGEsodium dodecyl ulphate polyacrylamide gel electropheresis

TCEPtrichloroethyl phosphate

IAAiodoacetamide

nanoUPLC-Q-Tofnanoultra performance liquid chromatrography-quadrupole-time of flight

PVDFpolyvinylidene difluoride

HandEhematoxylin and eosin

Rgnregucalcin

HGFhepatocyte growth factor

NF-Kbnuclear factor kappa-light-chain-enhancer of activated B cells

TNFtumor necrosis factor

ILinterleukin

LCliquid hromatography

MSmass spectrometry.

Electronic supplementary materialThe online version of this article doi:10.1186-1477-5956-7-48 contains supplementary material, which is available to authorized users.

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Author: Hongcui Cao - Jiong Yu - Wei Xu - Xiaofei Jia - Jinfeng Yang - Qiaoling Pan - Qiyi Zhang - Guoping Sheng - Jun Li - Xiaop

Source: https://link.springer.com/



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