Plexin-B1, glycodelin and MMP7 expression in the human fallopian tube and in the endometriumReport as inadecuate




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Reproductive Biology and Endocrinology

, 7:152

First Online: 29 December 2009Received: 11 August 2009Accepted: 29 December 2009

Abstract

BackgroundTo study the expression of Plexin-B1, Glycodelin, and MMP7 during the menstrual cycle in the endometrium and in the fallopian tube.

MethodsThe research included women undergoing hysterectomy, tubal sterilization or salpingo-oophoerectomy. Total RNA from endometrial and fallopian tube tissues was extracted using a total RNA isolation kit. Semi-quantitative RT-PCR was performed to examine mRNA relative expression.

ResultsPlexin-B1 expression in the endometrium was significantly higher on days 19 - 23 compared to days 12 - 14 1.166 +- 0.42 versus 0.523 +- 0.299, P < 0.005. In the fallopian tube the level of plexin-B1 did not change significantly throughout the menstrual cycle. Glycodelin expression was significantly higher on days 19 - 23 compared with days 12-14, both in the endometrium 0.819 +- 0.564 versus 0.072 +- 0.343, P < 0.05 and the fallopian tube 0.796 +- 0.196 versus 0.329 +- 0.398, P < 0.05. Although the level of MMP7 secretion was the highest in the secretory phase the difference from the proliferative phase did not reach statistical significance, neither in the endometrium nor in the fallopian tube. This could result from a lack of power.

ConclusionsIn the endometrium, both Glycodelin and Plexin-B1 are exhibiting a cyclic pattern suggesting a possible steroid regulation and a role in endometrial receptivity.

Electronic supplementary materialThe online version of this article doi:10.1186-1477-7827-7-152 contains supplementary material, which is available to authorized users.

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Author: Michal Amir - Shabtai Romano - Shlomit Goldman - Eliezer Shalev

Source: https://link.springer.com/



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