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Reproductive Biology and Endocrinology

, 6:27

First Online: 02 July 2008Received: 08 February 2008Accepted: 02 July 2008

Abstract

BackgroundThe placenta is an important site for iron metabolism in humans. It transfers iron from the mother to the fetus. One of the major iron transport proteins is transferrin, which is a blood plasma protein crucial for iron uptake. Its localization and expression may be one of the markers to distinguish placental dysfunction.

MethodsIn the experimental study we used antibody preparation, mass spectrometric analysis, biochemical and immunocytochemical methods for characterization of transferrin expression on the human choriocarcinoma cell line JAR JAR cells, placental lysates, and cryostat sections. Newly designed monoclonal antibody TRO-tf-01 to human transferrin was applied on human placentae from normal n = 3 and abnormal n = 9 pregnancies.

ResultsVariations of transferrin expression were detected in villous syncytiotrophoblast, which is in direct contact with maternal blood. In placentae from normal pregnancies, the expression of transferrin in the syncytium was significantly lower p < 0.001 when compared to placentae from abnormal ones gestational diabetes, pregnancy induced hypertension, drug abuse.

ConclusionThese observations suggest that in the case of abnormal pregnancies, the fetus may require higher levels of transferrin in order to prevent iron depletion due to the stress from the placental dysfunction.

Electronic supplementary materialThe online version of this article doi:10.1186-1477-7827-6-27 contains supplementary material, which is available to authorized users.

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Author: Alena Kralova - Marta Svetlikova - Jindrich Madar - Zdena Ulcova-Gallova - Antonin Bukovsky - Jana Peknicova

Source: https://link.springer.com/







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