New and old complex recombinant HIV-1 strains among patients with primary infection in 1996–2006 in France: The French ANRS CO06 primo cohort studyReport as inadecuate




New and old complex recombinant HIV-1 strains among patients with primary infection in 1996–2006 in France: The French ANRS CO06 primo cohort study - Download this document for free, or read online. Document in PDF available to download.

Retrovirology

, 5:69

First Online: 01 August 2008Received: 09 April 2008Accepted: 01 August 2008

Abstract

BackgroundPrevalence of HIV-1 non-B subtypes has increased overtime in patients diagnosed at the time of primary infection PHI in France. Our objective was to characterize in detail non-B strains which could not be genetically classified into the known subtypes-Circulating Recombinant Forms CRFs.

MethodsAmong 744 patients enrolled in the ANRS PRIMO Cohort since 1996, 176 23.7% were infected with HIV-1 non-B strains. The subtype-CRF could not be identified in RT for 15 2%. The V3-V5 env region was sequenced and 3 strains 04FR-KZS, 06FR-CRN, 04FR-AUK were full-length sequenced. Phylogenetic and bootscan analyses were used to characterize the mosaic structures.

ResultsAmong V3-V5 sequences, 6 were divergent A, 2 distantly related to E or D, 2 C, 1 B and 2 remained unclassified. 04FR-KZS, isolated in a Congolese woman infected in France, clustered with 2 previously described viruses from the Democratic Republic of Congo. They represent CRF27 cpx involving A-E-G-H-J-K-U subtypes. 06FR-CRN, isolated in a homosexual Caucasian patient, was a B-C-U recombinant involving a Brazilian C strain. 04FR-AUK, isolated in a Congolese patient infected in France, was a A-K-CRF09-U recombinant clustering from gag to vif with HIV-1 MAL. Others PHI were further observed in 2006–2007 with 1 KZS and 5 CRN-like viruses, suggesting their spread in France.

ConclusionThis study illustrates the increasing HIV-1 diversity in France associating new 06FR-CRN and old CRF27 cpx and -MAL-like- 04FR-AUK strains, which are rare in their region of origin but may have a possible founder effect in France. Our results strengthen the French guidelines recommending viro-epidemiological surveillance of HIV-1 diversity.

Electronic supplementary materialThe online version of this article doi:10.1186-1742-4690-5-69 contains supplementary material, which is available to authorized users.

Download fulltext PDF



Author: Pierre Frange - Julie Galimand - Nicole Vidal - Cécile Goujard - Christiane Deveau - Faouzi Souala - Martine Peeters - Lau

Source: https://link.springer.com/







Related documents