Intragenic transcriptional cis-activation of the human immunodeficiency virus 1 does not result in allele-specific inhibition of the endogenous geneReport as inadecuate




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Retrovirology

, 5:98

First Online: 04 November 2008Received: 06 October 2008Accepted: 04 November 2008

Abstract

BackgroundThe human immunodeficiency virus type 1 HIV-1 favors integration in active genes of host chromatin. It is believed that transcriptional interference of the viral promoter over the endogenous gene or vice versa might occur with implications in HIV-1 post-integrative transcriptional latency.

ResultsIn this work a cell line has been transduced with a HIV-based vector and selected for Tat-inducible expression. These cells were found to carry a single silent integration in sense orientation within the second intron of the HMBOX1 gene. The HIV-1 Tat transactivator induced the viral LTR and repressed HMBOX1 expression independently of vector integration. Instead, single-cell quantitative in situ hybridization revealed that allele-specific transcription of HMBOX1 carrying the integrated provirus was not affected by the transactivation of the viral LTR in cis.

ConclusionA major observation of the work is that the HIV-1 genome has inserted in genes that are also repressed by Tat and this could be an advantage for the virus during transcriptional reactivation. In addition, it has also been observed that transcription of the provirus and of the endogenous gene in which it is integrated may coexist at the same time in the same genomic location.

Electronic supplementary materialThe online version of this article doi:10.1186-1742-4690-5-98 contains supplementary material, which is available to authorized users.

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Author: Alex De Marco - Chiara Biancotto - Anna Knezevich - Paolo Maiuri - Chiara Vardabasso - Alessandro Marcello

Source: https://link.springer.com/







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