Chemical forms of calcium in Ca,Zn- and Ca,Cd-containing grains excreted by tobacco trichomesReport as inadecuate




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1 LGIT - Laboratoire de Géophysique Interne et Tectonophysique 2 ALS - Advanced Light Source ALS 3 Division of Forest Resources, College of Forest Sciences 4 POM - Optique et microscopies NEEL - Institut Néel

Abstract : Tobacco Nicotiana tabacum L. cv. Xanthi plants exposed to toxic levels of zinc and cadmium excrete metals through their leaf trichomes epidermal hairs as Zn,Ca- and Cd,Ca-containing grains. Little is known about the nature and formation mechanism of these precipitates. The chemical, crystalline, and non-crystalline compositions of individual grains produced by tobacco were studied by scanning electron microscopy coupled with energy dispersive X-ray analysis SEM-EDX, micro-X-ray diffraction μXRD and calcium K-edge micro X-ray absorption near edge structure μXANES spectroscopy. Zinc is predominantly incorporated in calcite, and cadmium in calcite and vaterite. Aragonite, which occurs occasionally, does not seem to contain trace metals. In addition to being precipitated in its three possible polymorphic forms, calcite, aragonite and vaterite, calcium also is speciated as amorphous CaCO3 and possibly organic Ca in some grains. Most often, a particular grain consists of two or more crystalline and non-crystalline phases. The observed variability of intra- and inter-grain elemental and phase composition suggests that this biomineralization process is not constrained by biological factors, but instead results from thermodynamically and kinetically controlled reactions. This study illustrates the potential of laterally resolved X-ray synchrotron radiation techniques μXRD and μXANES to study biomineralization and metal immobilization processes in plants.

keyword : biomineralization detoxification micro-XANES micro-XRD





Author: Geraldine Sarret - Marie-Pierre Isaure - Matthew Marcus - Emiko Harada - Yong-Eui Choi - Sébastien Pairis - Sirine Fakra - Alain

Source: https://hal.archives-ouvertes.fr/



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