Genetic subtraction profiling identifies genes essential for Arabidopsisreproduction and reveals interaction between the female gametophyte and the maternal sporophyteReport as inadecuate




Genetic subtraction profiling identifies genes essential for Arabidopsisreproduction and reveals interaction between the female gametophyte and the maternal sporophyte - Download this document for free, or read online. Document in PDF available to download.

Genome Biology

, 8:R204

First Online: 03 October 2007Received: 09 February 2007Revised: 10 September 2007Accepted: 03 October 2007

Abstract

BackgroundThe embryo sac contains the haploid maternal cell types necessary for double fertilization and subsequent seed development in plants. Large-scale identification of genes expressed in the embryo sac remains cumbersome because of its inherent microscopic and inaccessible nature. We used genetic subtraction and comparative profiling by microarray between the Arabidopsis thaliana wild-type and a sporophytic mutant lacking an embryo sac in order to identify embryo sac expressed genes in this model organism. The influences of the embryo sac on the surrounding sporophytic tissues were previously thought to be negligible or nonexistent; we investigated the extent of these interactions by transcriptome analysis.

ResultsWe identified 1,260 genes as embryo sac expressed by analyzing both our dataset and a recently reported dataset, obtained by a similar approach, using three statistical procedures. Spatial expression of nine genes for instance a central cell expressed trithorax-like gene, an egg cell expressed gene encoding a kinase, and a synergid expressed gene encoding a permease validated our approach. We analyzed mutants in five of the newly identified genes that exhibited developmental anomalies during reproductive development. A total of 527 genes were identified for their expression in ovules of mutants lacking an embryo sac, at levels that were twofold higher than in the wild type.

ConclusionIdentification of embryo sac expressed genes establishes a basis for the functional dissection of embryo sac development and function. Sporophytic gain of expression in mutants lacking an embryo sac suggests that a substantial portion of the sporophytic transcriptome involved in carpel and ovule development is, unexpectedly, under the indirect influence of the embryo sac.

AbbreviationsBLASTbasic local alignment search tool

coacoatlique mutant

dCHIPDNA-Chip Analyzer

FDRfalse discovery rate

gcRMAGC robust multi-array average

MASMicroArray Suite Affymetrix

RT-PCRreverse transcription polymerase chain reaction

splsporocyteless mutant

TAIRThe Arabidopsis Information Resource.

Electronic supplementary materialThe online version of this article doi:10.1186-gb-2007-8-10-r204 contains supplementary material, which is available to authorized users.

Amal J Johnston, Patrick Meier contributed equally to this work.

Download fulltext PDF



Author:

Source: https://link.springer.com/







Related documents