Specific antibody response of mice after immunization with COS-7 cell derived avian influenza virus H5N1 recombinant proteinsReport as inadecuate




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Journal of Immune Based Therapies and Vaccines

, 5:10

First Online: 03 October 2007Received: 07 March 2007Accepted: 03 October 2007

Abstract

To develop avian influenza H5N1 recombinant protein, the hemagglutinin HA, neuraminidase NA, matrix M, and non-structural NS1 of avian influenza H5N1 isolates from Thailand were engineered to be expressed in prokaryotic E. coli and mammalian cell COS-7 system. The plasmid pBAD-His and pSec-His were used as vectors for these inserted genes. Mice immunized with purified recombinant proteins at concentration 50–250 μg intramuscularly with Alum adjuvant at week 0, week 2, and week 3 showed a good immunogenicity measured by ELISA and neutralization assay. The HA and NS recombinant proteins produced in COS-7 cells can induce specific antibody titer detected by neutralization assay significantly higher than corresponding recombinant proteins produced in E. coli system. The antibody produced in immunized mice could neutralize heterologous avian influenza virus determined by micro-neutralization assay. This study shows that avian influenza virus H5N1 recombinant proteins produced in mammalian cell system were able to induce neutralizing antibody response.

Electronic supplementary materialThe online version of this article doi:10.1186-1476-8518-5-10 contains supplementary material, which is available to authorized users.

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Author: Navin Horthongkham - Tananun Srihtrakul - Niracha Athipanyasilp - Sontana Siritantikorn - Wannee Kantakamalakul - Yong Poovo

Source: https://link.springer.com/







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