Differential expression of viral PAMP receptors mRNA in peripheral blood of patients with chronic hepatitis C infectionReport as inadecuate




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BMC Infectious Diseases

, 7:136

First Online: 19 November 2007Received: 08 March 2007Accepted: 19 November 2007

Abstract

BackgroundPathogen-associated molecular patterns PAMP receptors play a key role in the early host response to viruses. In this work, we determined mRNA levels of two members of the Toll-like Receptors family, TLR3 and TLR7 and the helicase RIG-I, all of three recognizing viral RNA products, in peripheral blood of healthy donors and hepatitis C virus HCV patients, to observe if their transcripts are altered in this disease.

MethodsIFN-α, TLR3, TLR7 and RIG-I levels in peripheral blood from healthy controls n = 18 and chronic HCV patients n = 18 were quantified by real-time polymerase chain reaction.

ResultsOur results show that IFN-α, TLR3, TLR7 and RIG-I mRNA levels are significantly down-regulated in patients with chronic HCV infection when compared with healthy controls. We also found that the measured levels of TLR3 and TLR7, but not RIG-I, correlated significantly with those of IFN-α

ConclusionMonitoring the expression of RNA-sensing receptors like TLR3, TLR7 and RIG-I during the different clinical stages of infection could bring a new source of data about the prognosis of disease.

Electronic supplementary materialThe online version of this article doi:10.1186-1471-2334-7-136 contains supplementary material, which is available to authorized users.

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Author: Rafael Atencia - Francisco J Bustamante - Andrés Valdivieso - Arantza Arrieta - Marta Riñón - Alvaro Prada - Natalia Ma

Source: https://link.springer.com/







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