Broad network-based predictability of Saccharomyces cerevisiaegene loss-of-function phenotypesReport as inadecuate




Broad network-based predictability of Saccharomyces cerevisiaegene loss-of-function phenotypes - Download this document for free, or read online. Document in PDF available to download.

Genome Biology

, 8:R258

First Online: 05 December 2007Received: 24 July 2007Revised: 16 October 2007Accepted: 05 December 2007

Abstract

We demonstrate that loss-of-function yeast phenotypes are predictable by guilt-by-association in functional gene networks. Testing 1,102 loss-of-function phenotypes from genome-wide assays of yeast reveals predictability of diverse phenotypes, spanning cellular morphology, growth, metabolism, and quantitative cell shape features. We apply the method to extend a genome-wide screen by predicting, then verifying, genes whose disruption elongates yeast cells, and to predict human disease genes. To facilitate network-guided screens, a web server is available http:-www.yeastnet.org.

AbbreviationsAUCarea under the curve

CVcoefficient of variance

DIPDatabase of Interacting Proteins

FNfalse negative

FPfalse positive

GBAguilt-by-association

GOGene Ontology

MIPSMunich Information Center for Protein Sequences

OMIMOnline Mendelian Inheritance in Man

PICOProbabilistic Integrated Co-complex

ROCreceiver operating characteristic

SCMDS. cerevisiae Morphology Database

SGDSaccharomyces Genome Database

TNtrue negative

TPtrue positive.

Electronic supplementary materialThe online version of this article doi:10.1186-gb-2007-8-12-r258 contains supplementary material, which is available to authorized users.

Download fulltext PDF



Author: Kriston L McGary - Insuk Lee - Edward M Marcotte

Source: https://link.springer.com/



DOWNLOAD PDF




Related documents