Assessment of protein stability in cerebrospinal fluid using surface-enhanced laser desorption-ionization time-of-flight mass spectrometry protein profilingReport as inadecuate




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Clinical Proteomics

, Volume 2, Issue 1–2, pp 91–101

Abstract

Recent studies have evaluated proper acquisition and storage procedures for the use of serum or plasma for mass spectrometry MS-based proteomics. The present study examines the proteome stability of human cerebrospinal fluid CSF over time at 23°C room temperature and 4°C using surface-enhanced laser desorption-ionization time-of-flight MS. Data analysis revealed that statistically significant differences in protein profiles are apparent within 4 h at 23°C and between 6 and 8 h at 4°C. Inclusion of protease and phosphatase inhibitor cocktails into the CSF samples failed to significantly reduce proteome alterations over time. We conclude that MS-based proteomic analysis of CSF requires careful assessment of sample collection procedures for rapid and optimal sample acquisition and storage.

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Author: Srikanth Ranganathan - Anna Polshyna - Georgina Nicholl - James Lyons-Weiler - Robert Bowser

Source: https://link.springer.com/







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