Kinetic oxygen measurements by CVC96 in L-929 cell culturesReport as inadecuate




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Head and Face Medicine

, 2:6

First Online: 01 March 2006Received: 21 November 2005Accepted: 01 March 2006

Abstract

Generally animal and human cells use oxygen during their whole life. Consequently the oxygen use is a simple indicator to test the vitality of cells. When the vitality decreases by the delivery of toxic substances the decrease can be observed directly by the oxygen-use of the cells. To get fast information of the vitality of cells we have measured the O2-tension by testing a new model of a bioreactor, the Cell Vitality Checker 96 CVC96, in practical application. With this CVC96, soon a simple test will exist for the measurement of the oxygen use. In this respect the question had to be answered whether the use in the laboratory is easy and whether oxygen as a parameter in the vitality test can also be applied in future for problems in the field of material testing.

Electronic supplementary materialThe online version of this article doi:10.1186-1746-160X-2-6 contains supplementary material, which is available to authorized users.

Ulrich Plate, Tobias Polifke, Dieter Sommer, Jörg Wünnenberg and Hans-Peter Wiesmann contributed equally to this work.

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Author: Ulrich Plate - Tobias Polifke - Dieter Sommer - Jörg Wünnenberg - Hans-Peter Wiesmann

Source: https://link.springer.com/







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