Interleukin-1β and anaphylatoxins exert a synergistic effect on NGF expression by astrocytesReport as inadecuate




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Journal of Neuroinflammation

, 3:8

First Online: 04 April 2006Received: 14 December 2005Accepted: 04 April 2006

Abstract

C3a and C5a anaphylatoxins are proinflammatory polypeptides released during complement activation. They exert their biological activities through interaction with two G protein-coupled receptors named C3aR and C5aR, respectively. In the brain, these receptors are expressed on glial cells, and some recent data have suggested that anaphylatoxins could mediate neuroprotection. In this study, we used RT-PCR and ribonuclease protection assays RPA to investigate the role of anaphylatoxins on neurotrophin expression by the human glioblastoma cell line T98G and by rat astrocytes. Our data show that for both cell types, anaphylatoxins upregulate expression of NGF mRNA. This response depended on a G protein-coupled pathway since pre-treatment of cells with pertussis toxin PTX completely blocked NGF mRNA increases. This effect was anaphylatoxin-specific since pre-incubation with anti-C3a or anti-C5aR antibodies abolished the effects of C3a and C5a, respectively. The regulation of NGF mRNA by anaphylatoxins was not accompanied by translation into protein expression, but there was a significant synergic effect of anaphylatoxins-IL-1b costimulation. Our demonstration of involvement of anaphylatoxins in the NGF release process by astrocytes suggests that C3a and C5a could modulate neuronal survival in the CNS.

AbbreviationsCNScentral nervous system, NGF: nerve growth factor

PTXpertussis toxin, RPA: ribonuclease protection assay.

Electronic supplementary materialThe online version of this article doi:10.1186-1742-2094-3-8 contains supplementary material, which is available to authorized users.

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Author: Anne-christine Jauneau - Alexander Ischenko - Alexandra Chatagner - Magalie Benard - Philippe Chan - Marie-therese Schouft -

Source: https://link.springer.com/







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