Two dimensional VOPBA reveals laminin receptor LAMR1 interaction with dengue virus serotypes 1, 2 and 3Report as inadecuate




Two dimensional VOPBA reveals laminin receptor LAMR1 interaction with dengue virus serotypes 1, 2 and 3 - Download this document for free, or read online. Document in PDF available to download.

Virology Journal

, 2:25

First Online: 25 March 2005Received: 24 February 2005Accepted: 25 March 2005

Abstract

BackgroundThe search for the dengue virus receptor has generated many candidates often identified only by molecular mass. The wide host range of the viruses in vitro combined with multiple approaches to identifying the receptors has led to the notion that many receptors or attachment proteins may be involved and that the different dengue virus serotypes may utilize different receptors on the same cells as well as on different cell types.

ResultsIn this study we used sequential extraction of PS Clone D cell monolayers with the detergent β-octylglucopyranoside followed by sodium deoxycholate to prepare a cell membrane-rich fraction. We then used 2 dimensional 2D gel electrophoresis to separate the membrane proteins and applied a modified virus overlay protein binding assay VOPBA to show that dengue virus serotypes 1, 2 and 3 all interact with the 37 kDa-67 kDa laminin receptor LAMR1, a common non-integrin surface protein on many cell types.

ConclusionAt least 3 of the 4 dengue serotypes interact with the 37 kDa-67 kDa laminin receptor, LAMR1, which may be a common player in dengue virus-cell surface interaction.

Electronic supplementary materialThe online version of this article doi:10.1186-1743-422X-2-25 contains supplementary material, which is available to authorized users.

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Author: Phaik Hooi Tio - Wan Wui Jong - Mary Jane Cardosa

Source: https://link.springer.com/



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