DNA index determination with Automated Cellular Imaging System ACIS in Barretts esophagus: Comparison with CAS 200Report as inadecuate




DNA index determination with Automated Cellular Imaging System ACIS in Barretts esophagus: Comparison with CAS 200 - Download this document for free, or read online. Document in PDF available to download.

BMC Clinical Pathology

, 5:7

First Online: 12 August 2005Received: 08 February 2005Accepted: 12 August 2005

Abstract

BackgroundFor solid tumors, image cytometry has been shown to be more sensitive for diagnosing DNA content abnormalities aneuploidy than flow cytometry. Image cytometry has often been performed using the semi-automated CAS 200 system. Recently, an Automated Cellular Imaging System ACIS was introduced to determine DNA content DNA index, but it has not been validated.

MethodsUsing the CAS 200 system and ACIS, we compared the DNA index DI obtained from the same archived formalin-fixed and paraffin embedded tissue samples from Barrett-s esophagus related lesions, including samples with specialized intestinal metaplasia without dysplasia, low-grade dysplasia, high-grade dysplasia and adenocarcinoma.

ResultsAlthough there was a very good correlation between the DI values determined by ACIS and CAS 200, the former was 25% more sensitive in detecting aneuploidy. ACIS yielded a mean DI value 18% higher than that obtained by CAS 200 p < 0.001; paired t test. In addition, the average time required to perform a DNA ploidy analysis was shorter with the ACIS 30–40 min than with the CAS 200 40–70 min. Results obtained by ACIS gave excellent inter-and intra-observer variability coefficient of correlation >0.9 for both, p < 0.0001.

ConclusionCompared with the CAS 200, the ACIS is a more sensitive and less time consuming technique for determining DNA ploidy. Results obtained by ACIS are also highly reproducible.

List of abbreviations usedACISautomated cellular imaging system

DIDNA index

IODintegrated optical density

CVcoefficient of variation

BEBarrett-s esophagus

SIMspecialized intestinal metaplasia

LGDlow-grade dysplasia

HGDhigh-grade dysplasia

EACesophageal adenocarcinoma

Electronic supplementary materialThe online version of this article doi:10.1186-1472-6890-5-7 contains supplementary material, which is available to authorized users.

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Author: Qin Huang - Chenggong Yu - Michael Klein - James Fang - Raj K Goyal

Source: https://link.springer.com/







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