Expression profiling of mammalian microRNAs uncovers a subset of brain-expressed microRNAs with possible roles in murine and human neuronal differentiationReport as inadecuate




Expression profiling of mammalian microRNAs uncovers a subset of brain-expressed microRNAs with possible roles in murine and human neuronal differentiation - Download this document for free, or read online. Document in PDF available to download.

Genome Biology

, 5:R13

First Online: 16 February 2004Received: 17 October 2003Revised: 18 November 2003Accepted: 08 January 2004

Abstract

BackgroundThe microRNAs miRNAs are an extensive class of small noncoding RNAs 18 to 25 nucleotides with probable roles in the regulation of gene expression.
In Caenorhabditis elegans, lin-4 and let-7 miRNAs control the timing of fate specification of neuronal and hypodermal cells during larval development.
lin-4, let-7 and other miRNA genes are conserved in mammals, and their potential functions in mammalian development are under active study.

ResultsIn order to identify mammalian miRNAs that might function in development, we characterized the expression of 119 previously reported miRNAs in adult organs from mouse and human using northern blot analysis.
Of these, 30 miRNAs were specifically expressed or greatly enriched in a particular organ brain, lung, liver or skeletal muscle.
This suggests organ- or tissue-specific functions for miRNAs.
To test if any of the 66 brain-expressed miRNAs were present in neurons, embryonal carcinoma cells were treated with all-trans-retinoic acid to promote neuronal differentiation.
A total of 19 brain-expressed miRNAs including lin-4 and let-7 orthologs were coordinately upregulated in both human and mouse embryonal carcinoma cells during neuronal differentiation.
The mammalian ortholog of C.
elegans lin-28, which is downregulated by lin-4 in worms via 3- untranslated region binding, was also repressed during neuronal differentiation of mammalian embryonal carcinoma cells.
Mammalian lin-28 messenger RNAs contain conserved predicted binding sites in their 3- untranslated regions for neuron-expressed miR-125b a lin-4 ortholog, let-7a, and miR-218.

ConclusionsThe identification of a subset of brain-expressed miRNAs whose expression behavior is conserved in both mouse and human differentiating neurons implicates these miRNAs in mammalian neuronal development or function.

Electronic supplementary materialThe online version of this article doi:10.1186-gb-2004-5-3-r13 contains supplementary material, which is available to authorized users.

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Author: Lorenzo F Sempere - Sarah Freemantle - Ian Pitha-Rowe - Eric Moss - Ethan Dmitrovsky - Victor Ambros

Source: https://link.springer.com/



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