The DNMT3B C→T promoter polymorphism and risk of breast cancer in a British population: a case-control studyReport as inadecuate




The DNMT3B C→T promoter polymorphism and risk of breast cancer in a British population: a case-control study - Download this document for free, or read online. Document in PDF available to download.

Breast Cancer Research

, 6:R390

First Online: 19 May 2004Received: 05 February 2004Revised: 23 April 2004Accepted: 30 April 2004

Abstract

BackgroundGene promoter methylation is an important regulator of expression and is a key epigenetic factor in tumorigenesis. DNA methylation is mediated by DNA methyltransferases DNMTs, of which three active forms have been identified: DNMT1, DNM3A and DNMT3B. The C→T transition polymorphism C46359T in the promoter of the DNMT3B gene, which significantly increases transcriptional activity, has been postulated to increase the propensity for promoter-hypermethylation-mediated silencing of tumour suppressor genes.

MethodsTo determine the role of this polymorphism in breast cancer, we genotyped 352 cases and 258 controls from a British population. The breast cancer cases were selected on the basis of either an age at onset of less than 40 years, a family history of breast cancer irrespective of age at onset, or bilateral breast cancer diagnosed after 39 years of age irrespective of family history.

ResultsThe C allele was found to be more common in case subjects than in control subjects cases, 0.59; controls, 0.54 corresponding to a nominally significant increase in breast cancer risk to heterozygotes and CC homozygotes odds ratio 1.51, 95% confidence interval 1.01–2.25 in the dominant inheritance model.

ConclusionsOur findings contrast with those of a previous study, which showed that individuals carrying at least one T allele have a significantly increased risk of developing lung cancer. This discrepancy might be an artefact resulting from a chance variation, or it might point to differing influences of promoter hypermethylation in these cancer types.

Keywordsbreast cancer DNMT3B methylation polymorphism AbbreviationsCIconfidence interval

DNMTDNA methyltransferase

ORodds ratio

PCRpolymerase chain reaction.

Download fulltext PDF



Author: Karen G Montgomery - Mira CP Liu - Diana M Eccles - Ian G Campbell

Source: https://link.springer.com/



DOWNLOAD PDF




Related documents