Reannotation of the CELO genome characterizes a set of previously unassigned open reading frames and points to novel modes of host interaction in avian adenovirusesReport as inadecuate




Reannotation of the CELO genome characterizes a set of previously unassigned open reading frames and points to novel modes of host interaction in avian adenoviruses - Download this document for free, or read online. Document in PDF available to download.

BMC Bioinformatics

, 4:55

First Online: 07 November 2003Received: 02 September 2003Accepted: 07 November 2003

Abstract

BackgroundThe genome of the avian adenovirus Chicken Embryo Lethal Orphan CELO has two terminal regions without detectable homology in mammalian adenoviruses that are left without annotation in the initial analysis. Since adenoviruses have been a rich source of new insights into molecular cell biology and practical applications of CELO as gene a delivery vector are being considered, this genome appeared worth revisiting. We conducted a systematic reannotation and in-depth sequence analysis of the CELO genome.

ResultsWe describe a strongly diverged paralogous cluster including ORF-2, ORF-12, ORF-13, and ORF-14 with an ATPase-helicase domain most likely acquired from adeno-associated parvoviruses. None of these ORFs appear to have retained ATPase-helicase function and alternative functions e.g. modulation of gene expression during the early life-cycle must be considered in an adenoviral context. Further, we identified a cluster of three putative type-1-transmembrane glycoproteins with IG-like domains ORF-9, ORF-10, ORF-11 which are good candidates to substitute for the missing immunomodulatory functions of mammalian adenoviruses. ORF-16 located directly adjacent displays distant homology to vertebrate mono-ADP-ribosyltransferases. Members of this family are known to be involved in immuno-regulation and similiar functions during CELO life cycle can be considered for this ORF. Finally, we describe a putative triglyceride lipase merged ORF-18-19 with additional domains, which can be expected to have specific roles during the infection of birds, since they are unique to avian adenoviruses and Marek-s disease-like viruses, a group of pathogenic avian herpesviruses.

ConclusionsWe could characterize most of the previously unassigned ORFs pointing to functions in host-virus interaction. The results provide new directives for rationally designed experiments.

List of abbreviationsCELOChicken embryo lethal orphan virus

ORFOpen reading frame

FAdVFowl adenovirus

AAVAdeno-associated virus

TMTransmembrane region

IGImmunoglobulin

ARTADP-ribosyltransferase

MDVMarek-s disease like virus

Electronic supplementary materialThe online version of this article doi:10.1186-1471-2105-4-55 contains supplementary material, which is available to authorized users.

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Author: Stefan Washietl - Frank Eisenhaber

Source: https://link.springer.com/



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