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Reproductive Biology and Endocrinology

, 1:83

First Online: 07 November 2003Received: 11 July 2003Accepted: 07 November 2003

Abstract

Mammalian cloning by nuclear transfer from somatic cells has created new opportunities to generate animal models of genetic diseases in species other than mice. Although genetic mouse models play a critical role in basic and applied research for numerous diseases, often mouse models do not adequately reproduce the human disease phenotype. Cystic fibrosis CF is one such disease. Targeted ablation of the cystic fibrosis transmembrane conductance regulator CFTR gene in mice does not adequately replicate spontaneous bacterial infections observed in the human CF lung. Hence, several laboratories are pursuing alternative animal models of CF in larger species such as the pig, sheep, rabbits, and ferrets. Our laboratory has focused on developing the ferret as a CF animal model. Over the past few years, we have investigated several experimental parameters required for gene targeting and nuclear transfer NT cloning in the ferret using somatic cells. In this review, we will discuss our progress and the hurdles to NT cloning and gene-targeting that accompany efforts to generate animal models of genetic diseases in species such as the ferret.

Electronic supplementary materialThe online version of this article doi:10.1186-1477-7827-1-83 contains supplementary material, which is available to authorized users.

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Author: Ziyi Li - John F Engelhardt

Source: https://link.springer.com/



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