Human Neuroepithelial Cells Express NMDA ReceptorsReport as inadecuate

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BMC Neuroscience

, 4:28

First Online: 13 November 2003Received: 14 March 2003Accepted: 13 November 2003


L-glutamate, an excitatory neurotransmitter, binds to both ionotropic and metabotropic glutamate receptors. In certain parts of the brain the BBB contains two normally impermeable barriers: 1 cerebral endothelial barrier and 2 cerebral epithelial barrier. Human cerebral endothelial cells express NMDA receptors; however, to date, human cerebral epithelial cells neuroepithelial cells have not been shown to express NMDA receptor message or protein. In this study, human hypothalamic sections were examined for NMDA receptors NMDAR expression via immunohistochemistry and murine neuroepithelial cell line V1 were examined for NMDAR via RT-PCR and Western analysis. We found that human cerebral epithelium express protein and cultured mouse neuroepithelial cells express both mRNA and protein for the NMDA receptor. These findings may have important consequences for neuroepithelial responses during excitotoxicity and in disease.

KeywordsGlutamate NMDA Neuroepithelial Human Brain Electronic supplementary materialThe online version of this article doi:10.1186-1471-2202-4-28 contains supplementary material, which is available to authorized users.

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Author: Christopher D Sharp - M Fowler - TH JacksonIV - J Houghton - A Warren - A Nanda - I Chandler - B Cappell - A Long - A M


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