Potential antimutagenic activity of berberine, a constituent of Mahonia aquifoliumReport as inadecuate




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BMC Complementary and Alternative Medicine

, 2:2

First Online: 19 February 2002Received: 17 December 2001Accepted: 19 February 2002

Abstract

BackgroundAs part of a study aimed at developing new pharmaceutical products from natural resources, the purpose of this research was twofold: 1 to fractionate crude extracts from the bark of Mahonia aquifolium and 2 to evaluate the strength of the antimutagenic activity of the separate components against one of the common direct-acting chemical mutagens.

MethodsThe antimutagenic potency was evaluated against acridine orange AO by using Euglena gracilis as an eukaryotic test model, based on the ability of the test compound-fraction to prevent the mutagen-induced damage of chloroplast DNA.

ResultsIt was found that the antimutagenicity of the crude Mahonia extract resides in both bis-benzylisoquinoline BBI and protoberberine alkaloid fractions but only the protoberberine derivatives, jatrorrhizine and berberine, showed significant concentration-dependent inhibitory effect against the AO-induced chloroplast mutagenesis of E. gracilis. Especially berberine elicited, at a very low dose, remarkable suppression of the AO-induced mutagenicity, its antimutagenic potency being almost three orders of magnitude higher when compared to its close analogue, jatrorrhizine. Possible mechanisms of the antimutagenic action are discussed in terms of recent literature data. While the potent antimutagenic activity of the protoberberines most likely results from the inhibition of DNA topoisomerase I, the actual mechanisms for the BBI alkaloids is hard to be identified.

ConclusionsTaken together, the results indicate that berberine possesses promising antimutagenic-anticarcinogenic potential that is worth to be investigated further.

Electronic supplementary materialThe online version of this article doi:10.1186-1472-6882-2-2 contains supplementary material, which is available to authorized users.

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Author: Marta Čerňáková - Daniela Košt-álová - Viktor Kettmann - Miriam Plodová - Jaroslav Tóth - Ján Dřímal

Source: https://link.springer.com/







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