A role for the collagen I-III and MMP-1-13 genes in primary inguinal herniaReport as inadecuate




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BMC Medical Genetics

, 3:2

First Online: 19 February 2002Received: 04 September 2001Accepted: 19 February 2002

Abstract

BackgroundAbnormal collagen metabolism is thought to play an important role in the development of primary inguinal hernia. This is underlined by detection of altered collagen metabolism and structural changes of the tissue in patients with primary inguinal hernia. However, it is still unknown whether these alterations reflect a basic dysfunction of the collagen synthesis, or of collagen degradation.

MethodsIn the present study, we analysed type I and type III procollagen messenger ribonucleic acid mRNA and MMP-1 and MMP-13 mRNA in cultured fibroblasts from the skin of patients with primary inguinal hernia, and from patients without hernia controls by reverse transcription polymerase chain reaction RT-PCR and Northern Blot.

ResultsThe results indicated that the ratio of type I to type III procollagen mRNA was decreased in patients with primary hernia, showing significant differences as compared to controls p = 0.01. This decrease was mainly due to the increase of type III procollagen mRNA. Furthermore, RT-PCR analysis revealed that the expression of MMP-1 mRNA in patients with primary hernia is equivalent to that of controls p > 0.05. In addition, MMP-13 mRNA is expressed neither in patients with primary hernia nor in controls.

ConclusionWe concluded that abnormal change of type I and type III collagen mRNAs contribute to the development of primary inguinal hernia, whereas the expressions of MMP-1 and MMP-13 mRNA appears not to be involved in the development of primary inguinal hernia. Thus, the knowledge on the transcriptional regulation of collagen in patients with primary inguinal hernia may help to understand the pathogenesis of primary inguinal hernia, and implies new therapeutic strategies for this disease.

Electronic supplementary materialThe online version of this article doi:10.1186-1471-2350-3-2 contains supplementary material, which is available to authorized users.

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Author: Raphael Rosch - Uwe Klinge - Zhongyi Si - Karsten Junge - Bernd Klosterhalfen - Volker Schumpelick

Source: https://link.springer.com/







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