Altered prostanoid production by fibroblasts cultured from the lungs of human subjects with idiopathic pulmonary fibrosisReport as inadecuate

Altered prostanoid production by fibroblasts cultured from the lungs of human subjects with idiopathic pulmonary fibrosis - Download this document for free, or read online. Document in PDF available to download.

Respiratory Research

, 3:9

First Online: 23 February 2002Received: 29 May 2001Revised: 14 November 2001Accepted: 21 December 2001


BackgroundProstanoids are known to participate in the process of fibrogenesis. Because lung fibroblasts produce prostanoids and are believed to play a central role in the pathogenesis of idiopathic pulmonary fibrosis IPF, we hypothesized that fibroblasts HF cultured from the lungs of patients with IPF HF-IPF have an altered balance between profibrotic thromboxane TXA2 and antifibrotic prostacyclin PGI2 prostaglandins PGs when compared with normal human lung fibroblasts HF-NL.

MethodsWe measured inducible cyclooxygenase COX-2 gene and protein expression, and a profile of prostanoids at baseline and after IL-1β stimulation.

ResultsIn both HF-IPF and HF-NL COX-2 expression was undetectable at baseline, but was significantly upregulated by IL-1β. PGE2 was the predominant COX product in IL-1β-stimulated cells with no significant difference between HF-IPF and HF-NL 28.35 9.09–89.09 vs. 17.12 8.58–29.33 ng-10 cells-30 min, respectively; P = 0.25. TXB2 the stable metabolite of TXA2 production was significantly higher in IL-1β-stimulated HF-IPF compared to HF-NL 1.92 1.27–2.57 vs. 0.61 0.21–1.64 ng-10 cells-30 min, respectively; P = 0.007 and the ratio of PGI2 as measured by its stable metabolite 6-keto-PGF1α to TXB2 was significantly lower at baseline in HF-IPF 0.08 0.04–0.52 vs. 0.12 0.11–0.89 in HF-NL; P = 0.028 and with IL-1β stimulation 0.24 0.05–1.53 vs. 1.08 0.51–3.79 in HF-NL; P = 0.09.

ConclusionAn alteration in the balance of profibrotic and antifibrotic PGs in HF-IPF may play a role in the pathogeneses of IPF.

Keywordslung fibroblasts prostacyclin prostaglandins pulmonary fibrosis thromboxane AbbreviationsCOXcyclooxygenase

HFhuman fibroblasts

NLnormal lungs

IPFidiopathic pulmonary fibrosis






Download fulltext PDF

Author: Roberto Cruz-Gervis - Arlene A Stecenko - Ryszard Dworski - Kirk B Lane - James E Loyd - Richard Pierson - Gayle King -


Related documents