Proteasome-mediated degradation antagonizes critical levels of the apoptosis-inducing C1D proteinReport as inadecuate




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Cancer Cell International

, 2:12

First Online: 02 September 2002Received: 10 March 2002Accepted: 02 September 2002

Abstract

The C1D gene is expressed in a broad spectrum of mammalian cells and tissues but its product induces apoptotic cell death when exceeding a critical level. Critical levels are achieved in a fraction of cells by transient transfection with EGFP-tagged C1D expression constructs. However, transfected cells expressing sub-critical levels of C1DEGFP escape apoptotic cell death by activation of a proteasome-mediated rescue mechanism. Inhibition of the proteasome-dependent degradation of the C1DEGFP protein results in a parallel increase of the intracellular C1D level and in the fraction of apoptotic cells.

Electronic supplementary materialThe online version of this article doi:10.1186-1475-2867-2-12 contains supplementary material, which is available to authorized users.

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Author: Karsten Rothbarth - Hermann Stammer - Dieter Werner

Source: https://link.springer.com/







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