cagA and vacA in strains of Helicobacter pylori from ulcer and non-ulcerative dyspepsia patientsReport as inadecuate




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BMC Gastroenterology

, 2:20

First Online: 10 September 2002Received: 14 March 2002Accepted: 10 September 2002

Abstract

BackgroundThe cytotoxin associated gene A cagA, and the vacuolating cytotoxin gene A vacA of Helicobacter pylori have been associated to phenotypic characteristics of virulence. The objectives of this study were to detect the presence of cagA and to characterize the allelic variants of vacA in 63 strains of H. pylori isolated from colonized individuals with different clinical outcomes.

Methods38 strains were isolated from patients with non-ulcerative dyspepsia NUD and 25 were isolated from colonized individuals with peptic ulcers. The genotypic characterization was carried out utilizing PCR methodology. The presence of the cagA gene was detected using two set of primers from the middle conservative region of the cagA, and primers for the signal and middle region were used for the genotyping of vacA

ResultsThe presence of cagA showed similar rates in strains from peptic ulcers 60% and NUD patients 55%. Also similar was the prevalence of the allelic form s1 of vacA between the strains obtained from ulcers or NUD patients. However, the combination cagA+-vacA s1m1 was found more frequently among the H. pylori strains from peptic ulcer patients 52% than among strains isolated from NUD patients 26%, this difference was statistically significant p = 0.035.

ConclusionsThe presence of either cagA or the allelic variant s1 vacA alone do not have a predictive value as as a risk markers of severe gastric pathologies in the Chilean population. However, being infected by a H. pylori strain with the genotype cagA+-vacA s1m1 may be associated to an increased risk of acquiring a peptic ulcer disease.

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Author: Gustavo Faundez - Miriam Troncoso - Guillermo Figueroa

Source: https://link.springer.com/







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