Deficiency of Pten accelerates mammary oncogenesis in MMTV-Wnt-1 transgenic miceReport as inadecuate




Deficiency of Pten accelerates mammary oncogenesis in MMTV-Wnt-1 transgenic mice - Download this document for free, or read online. Document in PDF available to download.

BMC Molecular Biology

, 2:2

First Online: 23 January 2001Received: 18 December 2000Accepted: 23 January 2001

Abstract

BackgroundGermline mutations in the tumor suppressor PTEN predispose human beings to breast cancer, and genetic and epigenetic alterations of PTEN are also detected in sporadic human breast cancer. Germline Pten mutations in mice lead to the development of a variety of tumors, but mammary carcinomas are infrequently found, especially in mice under the age of six months.

ResultsTo better understand the role of PTEN in breast tumor development, we have crossed Pten heterozygous mice to MMTV-Wnt-1 transgenic mice that routinely develop ductal carcinomas in the mammary gland. Female Wnt-1 transgenics heterozygous for Pten developed mammary tumors earlier than Wnt-1 transgenics that were wild type for Pten. In most tumors arising in Pten heterozygotes, the Pten wild-type allele was lost, suggesting that cells lacking Pten function have a growth advantage over cells retaining a wild type allele. Tumors with LOH contained high levels of activated AKT-PKB, a downstream target of the PTEN-PI3K pathway.

ConclusionsAn animal model has been developed in which the absence of Pten collaborates with Wnt-1 to induce ductal carcinoma in the mammary gland. This animal model may be useful for testing therapies specific for tumors deregulated in the PTEN-PI3K-AKT pathway.

Electronic supplementary materialThe online version of this article doi:10.1186-1471-2199-2-2 contains supplementary material, which is available to authorized users.

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Author: Yi Li - Katrina Podsypanina - Xiufan Liu - Allison Crane - Lee K Tan - Ramon Parsons - Harold E Varmus

Source: https://link.springer.com/







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