In vitro production of peroxynitrite by haemocytes from marine bivalves: C-ELISA determination of 3-nitrotyrosine level in plasma proteins from Mytilus galloprovincialis and Crassostrea gigasReport as inadecuate




In vitro production of peroxynitrite by haemocytes from marine bivalves: C-ELISA determination of 3-nitrotyrosine level in plasma proteins from Mytilus galloprovincialis and Crassostrea gigas - Download this document for free, or read online. Document in PDF available to download.

BMC Immunology

, 2:1

First Online: 29 January 2001Received: 27 November 2000Accepted: 29 January 2001

Abstract

BackgroundPeroxynitrite is increasingly proposed as a contributor to defence system in marine bivalve. It can be formed by combinaison of superoxide and nitric oxide, and can react with tyrosine residues of proteins giving rise to 3-nitrotyrosine.

ResultsThe present article describes a competitive ELISA for the measurement of 3-nitrotyrosine contents of plasma proteins from marine bivalves by means of a monoclonal anti 3-nitrotyrosine antibody mouse IgG.

ConclusionsThis assay is sensitive enough to determine the amounts of 3-nitrotyrosine in plasma proteins from one animal only.

Using the C-ELISA, we have shown that the phagocytosis of zymosan particles increased the 3-nitrotyrosine levels of plasma proteins from mussel M. galloprovincialis and oyster C. gigas 5.8 and 7.5 times respectively.

Electronic supplementary materialThe online version of this article doi:10.1186-1471-2172-2-1 contains supplementary material, which is available to authorized users.

Download fulltext PDF



Author: Jean Torreilles - Bernard Romestand

Source: https://link.springer.com/







Related documents