Use of molecular beacons to verify that the serine hydroxymethyltransferase pseudogene SHMT-ps1 is unique to the order PrimatesReport as inadecuate




Use of molecular beacons to verify that the serine hydroxymethyltransferase pseudogene SHMT-ps1 is unique to the order Primates - Download this document for free, or read online. Document in PDF available to download.

Genome Biology

, 2:research0006.1

First Online: 29 January 2001Received: 22 November 2000Revised: 20 December 2000Accepted: 02 January 2001

Abstract

BackgroundThe serine hydroxymethyltransferase processed pseudogene SHMT-ps1 has been suggested to be unique to the order Primates because of the failure to amplify this sequence by PCR from genomic DNAs of any non-primate mammal species. Here -molecular beacon- probes specific to SHMT-ps1 were used in an attempt to verify this suggestion.

ResultsIn a search for SHMT-ps1-specific sequences using molecular beacons across a range of mammalian species, SHMT-ps1 was only found in primates. The molecular beacon assays also showed that SHMT-ps1 is present in both Old World and New World species but not among prosimians.

ConclusionsThese results suggest that SHMT-ps1 originated close to the origin of the Anthropoidea, some 40 to 50 million years ago.

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Author: Eric J Devor

Source: https://link.springer.com/







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