A human RNA polymerase II subunit is encoded by a recently generated multigene familyReport as inadecuate




A human RNA polymerase II subunit is encoded by a recently generated multigene family - Download this document for free, or read online. Document in PDF available to download.

BMC Molecular Biology

, 2:14

First Online: 30 November 2001Received: 08 August 2001Accepted: 30 November 2001

Abstract

BackgroundThe sequences encoding the yeast RNA polymerase II RPB subunits are single copy genes.

ResultsWhile those characterized so far for the human h RPB are also unique, we show that hRPB subunit 11 hRPB11 is encoded by a multigene family, mapping on chromosome 7 at loci p12, q11.23 and q22. We focused on two members of this family, hRPB11a and hRPB11b: the first encodes subunit hRPB11a, which represents the major RPB11 component of the mammalian RPB complex ; the second generates polypeptides hRPB11bα and hRPB11bβ through differential splicing of its transcript and shares homologies with components of the hPMS2L multigene family related to genes involved in mismatch-repair functions MMR. Both hRPB11a and b genes are transcribed in all human tissues tested. Using an inter-species complementation assay, we show that only hRPB11bα is functional in yeast. In marked contrast, we found that the unique murine homolog of RPB11 gene maps on chromosome 5 band G, and encodes a single polypeptide which is identical to subunit hRPB11a.

ConclusionsThe type hRPB11b gene appears to result from recent genomic recombination events in the evolution of primates, involving sequence elements related to the MMR apparatus.

Electronic supplementary materialThe online version of this article doi:10.1186-1471-2199-2-14 contains supplementary material, which is available to authorized users.

Sylvie Grandemange, Sophie Schaller contributed equally to this work.

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Author: Sylvie Grandemange - Sophie Schaller - Shigeru Yamano - Stanislas Du Manoir - George V Shpakovski - Marie-Geneviève Mattei

Source: https://link.springer.com/







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