RNA Control of HIV-1 Particle Size PolydispersityReport as inadecuate




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* Corresponding author 1 Phys-ENS - Laboratoire de Physique de l-ENS Lyon 2 CPBS - Centre d-études d-agents Pathogènes et Biotechnologies pour la Santé 3 Virologie humaine

Abstract : HIV-1, an enveloped RNA virus, produces viral particles that are known to be much more heterogeneous in size than is typical of non-enveloped viruses. We present here a novel strategy to study HIV-1 Viral Like Particles VLP assembly by measuring the size distribution of these purified VLPs and subsequent viral cores thanks to Atomic Force Microscopy imaging and statistical analysis. This strategy allowed us to identify whether the presence of viral RNA acts as a modulator for VLPs and cores size heterogeneity in a large population of particles. These results are analyzed in the light of a recently proposed statistical physics model for the self-assembly process. In particular, our results reveal that the modulation of size distribution by the presence of viral RNA is qualitatively reproduced, suggesting therefore an entropic origin for the modulation of RNA uptake by the nascent VLP.





Author: Cendrine Faivre-Moskalenko - Julien Bernaud - Audrey Thomas - Kevin Tartour - Yvonne Beck - Maksym Iazykov - John Danial - Morgan

Source: https://hal.archives-ouvertes.fr/



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