Cathepsin-D, a key protease in breast cancer, is up-regulated in obese mouse and human adipose tissue, and controls adipogenesis.: Cathepsin D, adipogenesis and obesityReport as inadecuate




Cathepsin-D, a key protease in breast cancer, is up-regulated in obese mouse and human adipose tissue, and controls adipogenesis.: Cathepsin D, adipogenesis and obesity - Download this document for free, or read online. Document in PDF available to download.

* Corresponding author 1 IRCM - Institut de recherche en cancérologie de Montpellier 2 UPS - Université de Toulouse, Institut de Médecine Moléculaire de Rangueil 3 IPBS - Institut de pharmacologie et de biologie structurale 4 I2MR - Institut de médecine moléculaire de Rangueil 5 Service de diabétologie, maladies métaboliques & maladies de la nutrition

Abstract : The aspartic protease cathepsin-D cath-D is overexpressed by human epithelial breast cancer cells and is closely correlated with poor prognosis in breast cancer. The adipocyte is one of the most prominent cell types in the tumor-microenvironment of breast cancer, and clinical studies have shown that obesity increases the incidence of breast cancer. Here, we provide the first evidence that cath-D expression is up-regulated in adipose tissue from obese human beings, as well as in adipocytes from the obese C57BI6-J mouse. Cath-D expression is also increased during human and mouse adipocyte differentiation. We show that cath-D silencing in 3T3-F442A murine preadipocytes leads to lipid-depleted cells after adipogenesis induction, and inhibits of the expression of PPARγ, HSL and aP2 adipocyte differentiation markers. Altogether, our findings demonstrate the key role of cath-D in the control of adipogenesis, and suggest that cath-D may be a novel target in obesity.

Keywords : cathepsin D adipocyte obesity adipogenesis differentiation cancer





Author: Olivier Masson - Christine Prébois - Danielle Derocq - Aline Meulle - Cédric Dray - Danielle Daviaud - Didier Quilliot - Philip

Source: https://hal.archives-ouvertes.fr/



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