Vascular density and endothelial cell expression of integrin alpha v beta 3 and E-selectin in murine tumours.Report as inadecuate

Vascular density and endothelial cell expression of integrin alpha v beta 3 and E-selectin in murine tumours. - Download this document for free, or read online. Document in PDF available to download.

* Corresponding author 1 Unité de pharmacologie chimique et génétique et d-imagerie

Abstract : The endothelial cell adhesion molecules, including the integrin alpha v beta 3 αvβ3 and E-selectin, are involved in the process of angiogenesis required for tumour growth, cell migration and metastasis. The purpose of this study was to assess and compare widely used tumour models to select the ones most suitable for angiogenesis research. Fifteen murine tumours were selected including melanoma B16, colon C26, C38, C51, mammary MA13, MA16, MA16-Adr, MA17, MA17-Adr, MA25, MA44, pancreatic PO2, PO3, Glasgow osteogenic sarcoma GOS and Lewis lung carcinoma LLC. The tumour vascular density, assessed using the platelet endothelial cell adhesion molecule 1 PECAM-1; CD31 immunostaining, revealed that B16 melanoma was poorly vascularized 15 % were the pancreatic tumours PO2 and PO3, the sarcoma GOS and the lung tumour LLC. The integrin αvβ3 and E-selectin, evaluated by immunohistology, showed that 7-15 tumours expressed the αvβ3 integrin which was homogeneously distributed on all tumour sections B16, C26, MA17-Adr, MA25, MA44, PO2, LLC. E-selectin was expressed in 4-15 tumours and its expression was restricted to the tumour periphery. Only 2-15 tumours B16 and C26 were shown to express both integrin αvβ3 and E-selectin. In conclusion, these data not only contribute to a better understanding of the tumour biology of murine tumours but can also guide the choice of appropriate models for antiangiogenic therapy, for selective drug delivery to tumours and the validation of tumour imaging modalities targeting these endothelial cell adhesion molecules.

Keywords : mouse tumors vascularization integrin alpha v beta 3 E-selectin melanoma colon mammary pancreas sarcoma lung

Author: Johanne Seguin - Céline Nicolazzi - Nathalie Mignet - Daniel Scherman - Guy Chabot -



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