Interaction of amino acid-functionalized silver nanoparticles and Candida albicans polymorphs: A deep-UV fluorescence imaging studyReport as inadecuate




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1 Vinca Institute of Nuclear Sciences 2 University of Belgrade Belgrade 3 Synchroton SOLEIL 4 Physiopathologie et traitement des maladies du foie

Abstract : The interaction of the tryptophan functionalized Ag nanoparticles and live Candida albicans cells was studied by synchrotron excitation deep-ultraviolet DUV fluorescence imaging at the DISCO beamline of Synchrotron SOLEIL.
DUV imaging showed that incubation of the fungus with functionalized nanopar- ticles results in significant increase in the fluorescence signal.
The analysis of the images revealed that the interaction of the nanoparticles with pseudohyphae polymorphs of the diploid fungus was less pro- nounced than in the case of yeast cells or budding spores.
The changes in the intensity of the fluorescence signals of the cells after incubation were followed in 327–353 nm and 370–410 nm spectral ranges that correspond to the fluorescence of tryptophan in non-polar and polar environment, respectively.
As a consequence of the environmental sensitivity of the silver-tryptophan fluorescent nanoprobe, we were able to determine the possible accumulation sites of the nanoparticles.
The analysis of the intensity decay kinetics showed that the photobleaching effects were more pronounced in the case of the functionalized nanoparticle treated cells.
The results of time-integrated emission in the mentioned spectral ranges sug- gested that the nanoparticles penetrate the cells, but that the majority of the nanoparticles attach to the cells’ surfaces.


Keywords : Fluorescence imaging C albicans tryptophan functionalization silver nanoparticles





Author: Radovan Dojčilović - Jelena Pajović - Dušan Božanić - Una Bogdanovic - Vesna Vodnik - Suzana Dimitrijević-Branković - Mio

Source: https://hal.archives-ouvertes.fr/



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