Ecological success of a group of Saccharomyces cerevisiae-Saccharomyces kudriavzevii hybrids in the northern european wine-making environment.Report as inadecuate




Ecological success of a group of Saccharomyces cerevisiae-Saccharomyces kudriavzevii hybrids in the northern european wine-making environment. - Download this document for free, or read online. Document in PDF available to download.

1 UHA - Université de Haute Alsace Mulhouse Colmar 2 SVQV - Santé de la vigne et qualité du vin 3 Université de Strasbourg 4 SPO - Sciences Pour l-Oenologie 5 USAMV 6 MICALIS - MICrobiologie de l-ALImentation au Service de la Santé humaine

Abstract : The hybrid nature of lager-brewing yeast strains has been known for 25 years; however, yeast hybrids have only recently been described in cider and wine fermentations. In this study, we characterized the hybrid genomes and the relatedness of the Eg8 industrial yeast strain and of 24 Saccharomyces cerevisiae-Saccharomyces kudriavzevii hybrid yeast strains used for wine making in France Alsace, Germany, Hungary, and the United States. An array-based comparative genome hybridization aCGH profile of the Eg8 genome revealed a typical chimeric profile. Measurement of hybrids DNA content per cell by flow cytometry revealed multiple ploidy levels 2n, 3n, or 4n, and restriction fragment length polymorphism analysis of 22 genes indicated variable amounts of S. kudriavzevii genetic content in three representative strains. We developed microsatellite markers for S. kudriavzevii and used them to analyze the diversity of a population isolated from oaks in Ardèche France. This analysis revealed new insights into the diversity of this species. We then analyzed the diversity of the wine hybrids for 12 S. cerevisiae and 7 S. kudriavzevii microsatellite loci and found that these strains are the products of multiple hybridization events between several S. cerevisiae wine yeast isolates and various S. kudriavzevii strains. The Eg8 lineage appeared remarkable, since it harbors strains found over a wide geographic area, and the interstrain divergence measured with a δμ2 genetic distance indicates an ancient origin. These findings reflect the specific adaptations made by S. cerevisiae-S. kudriavzevii cryophilic hybrids to winery environments in cool climates.

Keywords : Saccharomyces cerevisiae-S. kudriavzevii hybrids Saccharomyces cerevisiae Saccharomyces kudrivazevii diversity microsatellite typing wine Alsace Germany Hungary





Author: C. Erny - Pauline Raoult - Anne Alais - Gisele Butterlin - Pierre Delobel - F. Matei-Radoi - Serge Casaregola - Jean Luc Legras -

Source: https://hal.archives-ouvertes.fr/



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