In vivo spectroscopy and NMR metabolite fingerprinting approaches to connect the dynamics of photosynthetic and metabolic phenotypes in resurrection plant Haberlea rhodopensis during desiccation and recovery.Report as inadecuate




In vivo spectroscopy and NMR metabolite fingerprinting approaches to connect the dynamics of photosynthetic and metabolic phenotypes in resurrection plant Haberlea rhodopensis during desiccation and recovery. - Download this document for free, or read online. Document in PDF available to download.

* Corresponding author 1 Agricultural Academy 2 LPCV - Laboratoire de physiologie cellulaire végétale 3 Bulgarian Academy of Sciences 4 BGE - UMR S1038 - Laboratoire de Biologie à Grande Échelle 5 Sofia University 6 Institute of Organic Chemistry with Centre of Phytochemistry

Abstract : The resurrection plant Haberlea rhodopensis was used to study dynamics of drought response of photosynthetic machinery parallel with changes in primary metabolism. A relation between leaf water content and photosynthetic performance was established, enabling us to perform a non-destructive evaluation of the plant water status during stress. Spectroscopic analysis of photosynthesis indicated that, at variance with linear electron flow LEF involving photosystem PS I and II, cyclic electron flow around PSI remains active till almost full dry state at the expense of the LEF, due to the changed protein organization of photosynthetic apparatus. We suggest that, this activity could have a photoprotective role and prevent a complete drop in adenosine triphosphate ATP, in the absence of LEF, to fuel specific energy-dependent processes necessary for the survival of the plant, during the late states of desiccation. The NMR fingerprint shows the significant metabolic changes in several pathways. Due to the declining of LEF accompanied by biosynthetic reactions during desiccation, a reduction of the ATP pool during drought was observed, which was fully and quickly recovered after plants rehydration. We found a decline of valine accompanied by lipid degradation during stress, likely to provide alternative carbon sources for sucrose accumulation at late stages of desiccation. This accumulation, as well as the increased levels of glycerophosphodiesters during drought stress could provide osmoprotection to the cells.

Keywords : Aminoacids Resurrection plant Drought stress Photosynthesis Metabolism Plant Valine lipids degradation





Author: Petko Mladenov - Giovanni Finazzi - Richard Bligny - Daniela Moyankova - Diana Zasheva - Anne-Marie Boisson - Sabine Brugière -

Source: https://hal.archives-ouvertes.fr/



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