Snake venoms as a source of compounds modulating sperm physiology: Secreted phospholipases A2 from Oxyuranus scutellatus scutellatus impact sperm motility, acrosome reaction and in vitro fertilization in mice.: Snake venoms modulReport as inadecuate




Snake venoms as a source of compounds modulating sperm physiology: Secreted phospholipases A2 from Oxyuranus scutellatus scutellatus impact sperm motility, acrosome reaction and in vitro fertilization in mice.: Snake venoms modul - Download this document for free, or read online. Document in PDF available to download.

* Corresponding author 1 GIN - Grenoble Institut des Neurosciences 2 Plateforme de protéomique médicale 3 Institut d-oncologie-développement Albert Bonniot de Grenoble 4 Latoxan 5 ARCHAMPS TIMC-IMAG - Techniques de l-Ingénierie Médicale et de la Complexité - Informatique, Mathématiques et Applications Grenoble, UF de Biochimie et Génétique Moléculaire 6 INSERM U823, équipe 5 cibles diagnostiques ou thérapeutiques et vectorisation de drogues dans le cancer du poumon Institut d-oncologie-développement Albert Bonniot de Grenoble, Plateforme de protéomique médicale 7 IPMC - Institut de pharmacologie moléculaire et cellulaire 8 ARCHAMPS TIMC-IMAG - Techniques de l-Ingénierie Médicale et de la Complexité - Informatique, Mathématiques et Applications Grenoble, GIN - Grenoble Institut des Neurosciences

Abstract : The goal of this study was to identify new compounds from venoms able to modulate sperm physiology and more particularly sperm motility. For this purpose, we screened the effects of 16 snake venoms cleared of molecules higher than 15 kDa on sperm motility. Venoms rich in neurotoxins like those from Oxyuranus scutellatus scutellatus or Daboia russelii, were highly potent inhibitors of sperm motility. In contrast, venoms rich in myotoxins like those from Echis carinatus, Bothrops alternatus and Macrovipera lebetina, were inactive. From the main pharmacologically-active fraction of the Taipan snake O. scutellatus s., a proteomic approach allowed us to identify 16 different proteins, among which OS1 and OS2, two secreted phospholipases A2 sPLA2. Purified OS1 and OS2 mimicked the inhibitory effect on sperm motility and were likely responsible for the inhibitory effect of the active fraction. OS1 and OS2 triggered sperm acrosome reaction and induced lipid rearrangements of the plasma membrane. The catalytic activity of OS2 was required to modulate sperm physiology since catalytically inactive mutants had no effect. Finally, sperm treated with OS2 were less competent than control sperm to initiate in vitro normal embryo development. This is the first report characterizing sPLA2 toxins that modulate in vitro sperm physiology.

Keywords : snake venom secreted phospholipase A2 sperm motility acrosome reaction in vitro fertilization





Author: Jessica Escoffier - Morgane Couvet - Harold De Pomyers - Pierre Ray - Michel Seve - Gérard Lambeau - Michel De Waard - Christoph

Source: https://hal.archives-ouvertes.fr/



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