Cryopreservation of Bone Marrow Mononuclear Cells Alters Their Viability and Subpopulation Composition but Not Their Treatment Effects in a Rodent Stroke ModelReport as inadecuate




Cryopreservation of Bone Marrow Mononuclear Cells Alters Their Viability and Subpopulation Composition but Not Their Treatment Effects in a Rodent Stroke Model - Download this document for free, or read online. Document in PDF available to download.

Stem Cells International - Volume 2016 2016, Article ID 5876836, 7 pages -

Research ArticleStroke Program, Department of Neurology, McGovern Medical School, University of Texas Health Science Center, Houston, TX 77030, USA

Received 8 February 2016; Revised 2 May 2016; Accepted 22 May 2016

Academic Editor: Paulo Henrique Rosado de Castro

Copyright © 2016 Bing Yang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The systemic administration of autologous bone marrow BM derived mononuclear cells MNCs is under investigation as a novel therapeutic modality for the treatment of ischemic stroke. Autologous applications raise the possibility that MNCs could potentially be stored as a banked source. There have been no studies that investigate the effects of cryopreservation of BM-MNCs on their functional abilities in stroke models. In the present study, C57BL-6 mice were subjected to middle cerebral artery occlusion MCAo for 60 minutes and then divided into two treatment groups: fresh MNCs versus cryopreserved MNCs. BM-MNCs were collected at 22 hours after MCAo and were stored in liquid nitrogen for 12 months in cryopreserved MNCs group. BM-MNCs cellular viability, composition, and phenotype of the various subpopulations of mice BM-MNCs were evaluated by flow cytometry, and the behavioral recovery of stroke animals was tested with freshly harvested MNCs versus cryopreserved MNCs by corner test and ladder rung test. We found that long-term cryopreservation negatively impacts the cellular viability of bone marrow MNCs. Cryopreservation also alters the cellular composition of various subpopulations within the MNCs. However, despite the changes observed in cryopreserved cells, both fresh and frozen MNCs have similar beneficial effect on behavioral and histological outcomes.





Author: Bing Yang, Kaushik Parsha, Krystal Schaar, Nikunj Satani, Xiaopei Xi, Jaroslaw Aronowski, and Sean I. Savitz

Source: https://www.hindawi.com/



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